DETERMINATION OF MICRO QUANTITIES OF CITRIC ACID IN BIOLOGICAL FLUIDS

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A simplified photometric method for the determination of citric acid in biological fluids.

Studies on the oxidation and bromination of citric acid to pentabromoacetone were reported as early as 1847 by Cahours (1). This reaction has since formed bhe basis for many of the procedures for determining citric acid. A qualitative test by Stahre (2), reported in 1897, was adapted fol quantitative analysis by Kunz (3). Pucher, Sherman, and Vickery (4) described the first microphotometric met...

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Citric Acid Determination

Three types of methods have been proposed for the determination of citric acid in urine. Salant and Wise (1) used the unspecific mercuric sulfate reagent of Deniges (2) in a study of the toxicity of citrates. Thunberg (3) developed an enzymatic method employing a dehydrogenase said to be specific for citrate. The remaining methods depend on the so called pentabromoacetone method of Stahre (4), ...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1947

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(17)30841-4